High resolution DNA barcode library for European butterflies reveals continental patterns of mitochondrial genetic diversity
The examine of worldwide biodiversity will enormously profit from entry to complete DNA barcode libraries at continental scale, however such datasets are nonetheless very uncommon. Right here, we assemble the primary high-resolution reference library for European butterflies that gives 97% taxon protection (459 species) and 22,306 COI sequences. We estimate that we captured 62% of the entire haplotype range and present that almost all species possess a number of quite common haplotypes and plenty of uncommon ones.
Specimens within the dataset have a median 95.3% chance of being accurately recognized. Mitochondrial range displayed elevated haplotype richness in southern European refugia, establishing the generality of this key biogeographic sample for a complete taxonomic group. Fifteen % of the species are concerned in barcode sharing, however two thirds of those instances could mirror the necessity for additional taxonomic analysis. This dataset offers a novel useful resource for conservation and for finding out evolutionary processes, cryptic species, phylogeography, and ecology.
NEK1 deficiency impacts mitochondrial capabilities and the transcriptome of key DNA restore pathways
Earlier research have indicated necessary roles for NIMA-related kinase 1 (NEK1) in modulating DNA harm checkpoints and DNA restore capability. To broadly assess the contributions of NEK1 to genotoxic stress and mitochondrial capabilities, we characterised a number of related phenotypes of NEK1 CRISPR knockout (KO) and WT HAP1 cells. Our research revealed that NEK1 KO cells resulted in elevated apoptosis and hypersensitivity to the alkylator methyl methanesulfonate, the radiomimetic bleomycin, and UVC mild, but elevated resistance to the crosslinker cisplatin.
Mitochondrial functionalities have been additionally altered in NEK1 KO cells, with phenotypes of diminished mitophagy, elevated whole mitochondria, elevated ranges of reactive oxygen species, impaired advanced I exercise, and better quantities of mitochondrial DNA harm. RNA-seq transcriptome evaluation coupled with qRT-PCR research evaluating NEK1 KO cells with NEK1 overexpressing cells revealed that the expression of genes concerned in DNA restore pathways, similar to base excision restore, nucleotide excision restore, and double-strand break restore, are altered in a means which may affect genotoxin resistance. Collectively, our research underline and additional help that NEK1 serves as a hub signaling kinase in response to DNA harm, modulating DNA restore capability, mitochondrial exercise and cell destiny willpower.
Sparsely methylated mitochondrial cell free DNA launched from cardiomyocytes contributes to systemic inflammatory response accompanied by atrial fibrillation
Systemic irritation is assumed to be the consequence and the reason for atrial fibrillation (AF); nonetheless, the underlying mechanism stays unclear. We aimed to guage the extent of cell-free DNA (cfDNA) in sufferers with AF and AF mimicking fashions, and to light up its impression on irritation. Peripheral blood was obtained from 54 sufferers with AF and 104 non-AF controls, and cfDNA was extracted. We extracted whole cfDNA from conditioned medium after fast pacing to HL-1 cells.
Nuclear and mitochondrial DNA have been individually extracted and fragmented to simulate nuclear-cfDNA (n-cfDNA) and mitochondrial-cfDNA (mt-cfDNA). The AF group confirmed greater cfDNA focus than the non-AF group (12.6 [9.0-17.1] vs. 8.1 [5.3-10.8] [ng/mL], p < 0.001). The copy numbers of n-cfDNA and mt-cfDNA have been greater in AF teams than in non-AF teams; the distinction of mt-cfDNA was notably obvious (p = 0.011 and p < 0.001, respectively).
Administration of whole cfDNA and mt-cfDNA to macrophages considerably promoted IL-1β and IL-6 expression via TLR9, whereas n-cfDNA didn’t. Induction of cytokine expression by methylated mt-cfDNA was decrease than that by unmethylated mt-cfDNA. Collectively, AF was related to an elevated cfDNA degree, particularly mt-cfDNA. Sparsely methylated mt-cfDNA launched from cardiomyocytes could also be concerned in sterile systemic irritation accompanied by AF.
Skeletal muscle mitochondrial DNA copy quantity and mitochondrial DNA deletion mutation frequency as predictors of bodily efficiency in older women and men
Mitochondrial DNA (mtDNA) high quality and amount relate to 2 hallmarks of aging-genomic instability and mitochondrial dysfunction. Bodily efficiency depends on mitochondrial integrity and declines with age, but the interactions between mtDNA amount, high quality, and bodily efficiency are unclear. Utilizing a validated digital PCR assay particular for mtDNA deletions, we examined the speculation that skeletal muscle mtDNA deletion mutation frequency (i.e., a measure of mtDNA high quality) or mtDNA copy quantity predicts bodily efficiency in older adults.
Complete DNA was remoted from vastus lateralis muscle biopsies and used to quantitate mtDNA copy quantity and mtDNA deletion frequency by digital PCR. The biopsies have been obtained from a cross-sectional cohort of 53 adults aged 50 to 86 years. Earlier than the biopsy process, bodily efficiency measurements have been collected, together with VO2max, modified bodily efficiency take a look at rating, 6-min stroll distance, gait velocity, grip power, and whole lean and leg mass.
Linear regression fashions have been used to guage the relationships between age, intercourse, and the outcomes. We discovered that mtDNA deletion mutation frequency elevated exponentially with advancing age. On common from ages 50 to 86, deletion frequency elevated from 0.008 to 0.15%, an 18-fold improve. Females could have decrease deletion frequencies than males at older ages. We additionally measured declines in VO2max and mtDNA copy quantity with age in each sexes.
The mtDNA deletion frequency measured from single skeletal muscle biopsies predicted 13.3% of the variation in VO2max. Copy quantity defined 22.6% of the variation in mtDNA deletion frequency and 10.4% of the lean mass variation. We discovered predictive relationships between age, mtDNA deletion mutation frequency, mtDNA copy quantity, and bodily efficiency.
Bacillus anthracis antibody (Spore) |
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| 10-B108A | Fitzgerald | 500 ug | EUR 381 |
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Description: Mouse monoclonal Bacillus anthracis antibody (Spore) |
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Bacillus anthracis antibody (Spore) |
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| 10-B108B | Fitzgerald | 500 ug | EUR 381 |
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Description: Mouse monoclonal Bacillus anthracis antibody (Spore) |
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Bacillus anthracis (Anthrax) antibody |
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| 10R-B106a | Fitzgerald | 1 mg | EUR 568 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis (Anthrax) antibody |
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| 10R-B106b | Fitzgerald | 1 mg | EUR 568 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis (Anthrax) antibody |
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| 10R-B106c | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis (Anthrax) antibody |
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| 10R-B106d | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis (Anthrax) antibody |
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| 10R-B106e | Fitzgerald | 1 mg | EUR 469 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis (Anthrax) antibody |
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| 10R-B106f | Fitzgerald | 1 mg | EUR 568 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis (Anthrax) antibody |
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| 20-BR04 | Fitzgerald | 500 ug | EUR 358 |
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Description: Rabbit polyclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis RT PCR kit |
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| RTq-H504-100D | Bioingentech | 100T | EUR 717 |
Bacillus anthracis RT PCR kit |
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| RTq-H504-150D | Bioingentech | 150T | EUR 808 |
Bacillus anthracis RT PCR kit |
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| RTq-H504-50D | Bioingentech | 50T | EUR 598 |
Bacillus anthracis Thymidylate kinase (tmk) |
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| 1-CSB-EP007226BQG | Cusabio |
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Description: Recombinant Bacillus anthracis Thymidylate kinase(tmk) expressed in E.coli |
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Bacillus anthracis antibody (Lethal Factor) |
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| 10-7841 | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis antibody (Lethal Factor) |
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Bacillus anthracis antibody (Lethal Factor) |
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| 10-7842 | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis antibody (Lethal Factor) |
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Bacillus anthracis antibody (Lethal Factor) |
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| 10-B107A | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis antibody (Lethal Factor) |
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Bacillus anthracis antibody (Lethal Factor) |
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| 10-B107B | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis antibody (Lethal Factor) |
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Bacillus anthracis (Anthrax) antibody (Spore) |
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| 10C-CR9004M3 | Fitzgerald | 100 ug | EUR 503 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody (Spore) |
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Bacillus anthracis (Anthrax) antibody (Spore) |
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| 10C-CR9004M4 | Fitzgerald | 100 ug | EUR 503 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody (Spore) |
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Bacillus anthracis antibody (Protective Antigen) |
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| 70-1007 | Fitzgerald | 1 mg | EUR 367 |
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Description: Polyclonal Bacillus anthracis antibody (Protective Antigen), Anti-Bacillus anthracis antibody (Protective Antigen), Bacillus anthracis PA antibody, Anthrax PA antibody, Bacillus anthracis Protective Antigen antibody, Anthrax Protective Antigen antibody |
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Bacillus anthracis antibody (Lethal Factor) |
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| 70-1008 | Fitzgerald | 1 mg | EUR 367 |
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Description: Polyclonal Bacillus anthracis antibody (Lethal Factor), Anti-Bacillus anthracis antibody (Lethal Factor), Bacillus anthracis LF antibody, Anthrax LF antibody, Bacillus anthracis Lethal Factor antibody, Anthrax Lethal Factor antibody |
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Bacillus anthracis antibody (Edema Factor) |
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| 70-1009 | Fitzgerald | 1 mg | EUR 438 |
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Description: Polyclonal Bacillus anthracis antibody (Edema Factor), Anti-Bacillus anthracis antibody (Edema Factor), Bacillus anthracis EF antibody, Anthrax EF antibody, Bacillus anthracis Edema Factor antibody, Anthrax Edema Factor antibody |
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Bacillus anthracis (Anthrax) antibody (Spore) |
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| 10R-B108a | Fitzgerald | 1 mg | EUR 651 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody (Spore) |
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Bacillus anthracis (Anthrax) antibody (Spore) |
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| 10R-B108b | Fitzgerald | 1 mg | EUR 651 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody (Spore) |
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Recombinant Bacillus Anthracis Edema Factor (S447N Mutant) |
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| VAng-Lsx1539-500g | Creative Biolabs | 500 µg | EUR 2665 |
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Description: Bacillus Anthracis Edema Factor (S447N Mutant), recombinant protein. |
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Recombinant Bacillus Anthracis Lethal Factor (E687C Mutant) |
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| VAng-Lsx1541-100g | Creative Biolabs | 100 µg | EUR 822 |
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Description: Bacillus Anthracis Lethal Factor (E687C Mutant), recombinant protein. |
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Colwellia psychrerythraea DNA polymerase IV (dinB) |
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| 1-CSB-EP676401CBAB | Cusabio |
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Description: Recombinant Colwellia psychrerythraea DNA polymerase IV(dinB) expressed in E.coli |
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Colwellia psychrerythraea DNA polymerase IV (dinB) |
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| 1-CSB-EP676401CBABe1 | Cusabio |
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Description: Recombinant Colwellia psychrerythraea DNA polymerase IV(dinB) expressed in E.coli |
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Bacillus anthracis One-Step PCR kit |
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| Oneq-H504-100D | Bioingentech | 100T | EUR 866 |
Bacillus anthracis One-Step PCR kit |
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| Oneq-H504-150D | Bioingentech | 150T | EUR 981 |
Bacillus anthracis One-Step PCR kit |
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| Oneq-H504-50D | Bioingentech | 50T | EUR 718 |
Bacillus anthracis (Anthrax) antibody (Lethal Factor) |
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| 10-B03A | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody (Lethal Factor) |
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Bacillus anthracis (Anthrax) antibody (Lethal Factor) |
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| 10-B03B | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody (Lethal Factor) |
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Bacillus anthracis (Anthrax) antibody (Protective Antigen) |
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| 10C-CR9004M1 | Fitzgerald | 100 ug | EUR 408 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody (Protective Antigen) |
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Bacillus anthracis (Anthrax) antibody (Protective Antigen) |
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| 10C-CR9004M2 | Fitzgerald | 100 ug | EUR 408 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody (Protective Antigen) |
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Bacillus anthracis antibody (Lethal Factor) (biotin) |
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| 61R-B107bBT | Fitzgerald | 1 mg | EUR 976 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody (Lethal Factor) (biotin) |
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Bacillus anthracis (Anthrax) antibody (Lethal Factor) |
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| 10R-B107a | Fitzgerald | 1 mg | EUR 568 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody (Lethal Factor) |
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Bacillus anthracis (Anthrax) antibody (Lethal Factor) |
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| 10R-B107b | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody (Lethal Factor) |
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Bacillus Anthracis (Anthrax) protein (Protective Antigen) |
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| 30R-AA015 | Fitzgerald | 100 ug | EUR 993 |
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Description: Purified recombinant Bacillus Anthracis (Anthrax) protein (Protective Antigen) |
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Mouse antibody for Bacillus anthracis protective ag. |
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| 7821 | Virostat | 100 ug | EUR 321.88 |
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Description: This is purified Mouse monoclonal antibody against Bacillus anthracis protective ag for WB, ELISA. |
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Mouse antibody for Bacillus anthracis protective ag. |
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| 7825 | Virostat | 100 ug | EUR 321.88 |
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Description: This is purified Mouse monoclonal antibody against Bacillus anthracis protective ag for WB, ELISA. |
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Mouse antibody for Bacillus anthracis spore ag. |
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| 7826 | Virostat | 100 ug | EUR 354.5 |
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Description: This is purified Mouse monoclonal antibody against Bacillus anthracis spore ag for WB, ELISA. |
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Mouse antibody for Bacillus anthracis spore ag. |
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| 7827 | Virostat | 100 ug | EUR 354.5 |
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Description: This is purified Mouse monoclonal antibody against Bacillus anthracis spore ag for WB, ELISA. |
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Inactivated Bacillus Anthracis Protective Antigen PA 20 |
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| VAng-Lsx1534-50g | Creative Biolabs | 50 µg | EUR 1047 |
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Description: Bacillus Anthracis Protective Antigen PA 20, native protein from B. anthracis. |
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Inactivated Bacillus Anthracis Protective Antigen PA 63 |
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| VAng-Lsx1535-500g | Creative Biolabs | 500 µg | EUR 1495 |
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Description: Bacillus Anthracis Protective Antigen PA 63, native protein from B. anthracis. |
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Mouse Anti-Bacillus anthracis Lethal Factor Monoclonal Antibody |
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| DMAB3020 | Creative Diagnostics | 1 mg | EUR 955 |
Mouse Anti-Bacillus anthracis Lethal Factor Monoclonal Antibody |
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| DMAB3023 | Creative Diagnostics | 1 mg | EUR 955 |
Mouse Anti-Bacillus anthracis Protective Antigen Monoclonal Antibody |
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| DMAB3024 | Creative Diagnostics | 1 mg | EUR 903 |
Mouse Anti-Bacillus anthracis Protective Antigen Monoclonal Antibody |
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| DMAB3028 | Creative Diagnostics | 1 mg | EUR 955 |
Mouse Anti-Bacillus anthracis Protective Antigen Monoclonal Antibody |
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| DMAB3029 | Creative Diagnostics | 1 mg | EUR 955 |
Mouse Anti-Bacillus anthracis Protective Antigen Monoclonal Antibody |
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| DMAB3031 | Creative Diagnostics | 1 mg | EUR 903 |
Mouse Anti-Bacillus anthracis Spore Antigen Monoclonal Antibody |
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| DMAB3033-05mg | Creative Diagnostics | 0.5 mg | EUR 969 |
Mouse Anti-Bacillus anthracis Spore Antigen Monoclonal Antibody |
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| DMAB3033-1mg | Creative Diagnostics | 1 mg | EUR 1469 |
Mouse Anti-Bacillus anthracis Spore Antigen Monoclonal Antibody |
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| DMAB3034-05mg | Creative Diagnostics | 0.5 mg | EUR 969 |
Mouse Anti-Bacillus anthracis Spore Antigen Monoclonal Antibody |
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| DMAB3034-1mg | Creative Diagnostics | 1 mg | EUR 1469 |
Taq DNA Polymerase (Recombinant) |
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| abx073521-10000U | Abbexa | 10000 U | EUR 592 |
Taq DNA Polymerase (Recombinant) |
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| abx073521-1000U | Abbexa | 1000 U | EUR 230 |
Taq DNA Polymerase (Recombinant) |
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| abx073521-3000U | Abbexa | 3000 U | EUR 328 |
Pfu DNA Polymerase (Recombinant) |
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| abx074017-100U | Abbexa | 100 U | EUR 328 |
Pfu DNA Polymerase (Recombinant) |
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| abx074017-2500U | Abbexa | 2500 U | EUR 1274 |
Pfu DNA Polymerase (Recombinant) |
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| abx074017-500U | Abbexa | 500 U | EUR 439 |
Recombinant Taq DNA Polymerase |
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| 7-03634 | CHI Scientific | 1,000U | Ask for price |
Recombinant Taq DNA Polymerase |
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| 7-03635 | CHI Scientific | 3,000U | Ask for price |
Recombinant Taq DNA Polymerase |
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| 7-03636 | CHI Scientific | 10,000U | Ask for price |
Recombinant Pfu DNA Polymerase |
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| 7-03322 | CHI Scientific | 100U | Ask for price |
Recombinant Pfu DNA Polymerase |
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| 7-03323 | CHI Scientific | 500U | Ask for price |
Recombinant Pfu DNA Polymerase |
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| 7-03324 | CHI Scientific | 2500U | Ask for price |
DNA Polymerase |
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| ABD3082 | Lifescience Market | 100 ug | EUR 438 |
DNA Polymerase |
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| ABF5235 | Lifescience Market | 100 ug | EUR 438 |
Human Kinase Library IV |
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| HKIN-IV | Real Time Primers | 1 set | EUR 450 |
Polymerase (DNA directed), beta (Recombinant) |
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| 20-abx073237 | Abbexa |
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Taq Plus DNA Polymerase (Recombinant) |
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| abx073522-2500U | Abbexa | 2.500 U | EUR 592 |
Taq Plus DNA Polymerase (Recombinant) |
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| abx073522-250U | Abbexa | 250 U | EUR 230 |
Taq Plus DNA Polymerase (Recombinant) |
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| abx073522-750U | Abbexa | 750 U | EUR 328 |
Polymerase (DNA directed), lambda (Recombinant) |
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| 20-abx073724 | Abbexa |
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Recombinant Taq Plus DNA Polymerase |
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| 7-03631 | CHI Scientific | 250U | Ask for price |
Recombinant Taq Plus DNA Polymerase |
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| 7-03632 | CHI Scientific | 750U | Ask for price |
Recombinant Taq Plus DNA Polymerase |
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| 7-03633 | CHI Scientific | 2,500U | Ask for price |
Recombinant (E.Coli) Taq DNA Polymerase |
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| RP-394 | Alpha Diagnostics | 1000 IU | EUR 164 |
DNA Polymerase beta Recombinant Protein |
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| 91-139 | ProSci | 0.05 mg | EUR 542.75 |
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Description: Human DNA polymerase beta is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase beta . |
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DNA Ligase IV recombinant monoclonal antibody |
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| A5820 | Bimake | 100ul X 3 | EUR 595 |
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Description: A recombinant monoclonal antibody from rabbit against human DNA Ligase IV for WB, IHC, IF,ELISA |
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Taq DNA Polymerase |
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| BA00104 | Bioatlas | 1000U | EUR 123 |
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Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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Taq DNA Polymerase |
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| BA00105 | Bioatlas | 2500U | EUR 232 |
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Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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HotTaq DNA Polymerase |
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| BA00203 | Bioatlas | 500U | EUR 145 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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HotTaq DNA Polymerase |
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| BA00204 | Bioatlas | 1000U | EUR 239 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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HotTaq DNA Polymerase |
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| BA00205 | Bioatlas | 2500U | EUR 522 |
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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RedTaq DNA Polymerase |
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| BA00303 | Bioatlas | 500U | EUR 123 |
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Description: High quality RedTaq polymerase for different PCR variations and downstream applications. |
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RedTaq DNA Polymerase |
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| BA00304 | Bioatlas | 1000U | EUR 196 |
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Description: High quality RedTaq polymerase for different PCR variations and downstream applications. |
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RedTaq DNA Polymerase |
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| BA00305 | Bioatlas | 2500U | EUR 413 |
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Description: High quality RedTaq polymerase for different PCR variations and downstream applications. |
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Pfu DNA Polymerase |
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| BA00503 | Bioatlas | 500U | EUR 145 |
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Description: High quality Pfu polymerase for different PCR variations and downstream applications. |
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Pfu DNA Polymerase |
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| BA00504 | Bioatlas | 1000U | EUR 239 |
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Description: High quality Pfu polymerase for different PCR variations and downstream applications. |
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Pfu DNA Polymerase |
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| BA00505 | Bioatlas | 2500U | EUR 522 |
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Description: High quality Pfu polymerase for different PCR variations and downstream applications. |
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These information are according to a job for mitochondrial operate and genome integrity in sustaining bodily efficiency with age. Analyses of mtDNA high quality and amount in bigger cohorts and longitudinal research may prolong our understanding of the significance of mitochondrial DNA in human getting older and longevity.
